BioVendor Antibody Service

We have the facilities to breed experimental animals, experience to immunize animals and raise high affinity and specific polyclonal antibodies, expertise to develop monoclonal antibodies in cell fusions, and the technology enabling us to purify the antibodies. BioVendor’s monoclonal and polyclonal antibodies have become the backbone of many of our as well as other companies’ immunodiagnostics. Our antibodies applied in western blotting, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, and ELISA have helped numerous scientists obtain original data that have resulted in many publications.

The process starts with the immunization of experimental animals using the antigen supplied by a customer and finishes with a lyophilized antibody delivered to the client. Our standard service packages for polyclonal or monoclonal antibody productions are described in the Service Workflow charts.

The affinity and specificity of the prepared antibodies are mainly determined by the antigen applied, the number of desirable epitopes it presents and its purity. The use of BioVendor’s proven protocols and number of immunized animals minimize naturally random character of the immune response and guarantee the best possible results.

Features in brief:

Rabbit polyclonal antibody production (10-week protocol)
Sheep polyclonal antibody production (24-week protocol)
Polyclonal antibodies are affinity purified on an immunizing antigen column
Mouse monoclonal antibody production (24-week protocol)
Monoclonal antibodies are affinity purified on a Protein G column
Production and purification of monoclonal antibodies from customer’s hybridomas in ascites fluid of mice (8-week protocol)

We offer:

consulting and customization of the processes
customer control of the process (customer will decide to stop or go to the next step)
preparation of immunization recombinant antigen in E.coli using BioVendor’s service BIRDS
monoclonal antibody production and purification using hybridoma cells supplied by a customer
customization of antibody characterization employing the most advanced methods

We guarantee:

the use of sophisticated low-antigen-amountrequiring immunization protocols
production of the antibodies meeting standard quality demands
over 95 % purity of the product
easy conjugation formulation – azide free and NH2-group free product
reporting on regular basis
fast response to client’s demands
the whole process is performed in facilities located in the EU countries, fulfilling all strict regulations of the EU legislation
favorable pricing