Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
5 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
1 - 32 ng/ml
Limit of Detection
0.125 ng/ml
Intra-assay (Within-Run)
n = 8,
CV = 1.9%
Inter-assay (Run-to-Run)
n = 8,
CV =5.0%
Spiking Recovery
101.5%
Dilution Linearity
110.2%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures specifically Lipopolysaccharide Binding Protein
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Immune Response, Infection and Inflammation, Sepsis
Summary
Lipopolysaccharide binding protein (LBP, LPS-binding protein) is a serum glycoprotein belonging to the family of lipid-binding proteins. LBP is synthesized by hepatocytes and intestinal epithelial cells. Serum concentrations of LBP range between 5 and 10 ug/ml during homeostasis, increasing up to 200 ug/ml during the acute-phase response in the course of infection.
LBP expression and function are strongly associated with recognition and control of bacterial infection. LBP is an acute-phase reactant. As a class I acute-phase protein, LBP is induced by pro-inflammatory cytokines such as interleukins 1 and 6, tumor necrosis factor-alpha (TNF-α), and glucocorticoid hormones in liver and in non-hepatic tissues such as the gut and the lung.
LPS is released from CD14 in the lipid bilayer and binds to a complex of receptors including Toll-like receptor 4 (TLR-4) to initiate intracellular signaling cascades and transcription of genes mediated through NF-κB.
If dysregulated, these host reactions can have inadvertent outcomes such as severe sepsis, septic shock, or systemic inflammatory response syndrome (SIRS).
LBP binds LPS in various pathologic states including obesity and insulin resistance.
Instructions for Use (RUO)
Instructions for Use (RUO)
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