Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin
Sample Requirements
35 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
2–100 ng/ml
Limit of Detection
Analytical Limit of Detection is calculated from the real Leptin receptor values in wells and is 0.04 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 7.2%
Inter-assay (Run-to-Run)
n = 7; CV = 8.0%
Spiking Recovery
88,60%
Dilution Linearity
107,90%
Crossreactivity
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- goat Non-detectable
- horse Non-detectable
- hamster Not tested
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- human Yes
Features
- For research use only !
- The total assay time is less than four hours.
- The kit measures total serum Leptin receptor.
- Standard is recombinat protein based.
- Quality Controls are human serum based.
- Components of the kit are ready-to-use (with the exception of Wash Solution and Quality Controls).
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction
Summary
Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816 amino acid residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB-R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
MSDS (RUO)
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