Adipocyte Fatty Acid Binding Protein (AFABP, FABP4) Human,
Goat Polyclonal Antibody

Source of Antigen

E. coli

Host

Goat

Preparation

The antibody was raised in goat by immunization with the recombinant Human FABP4.

Amino Acid Sequence

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human FABP4.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C.Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References

• Prinsen CF, de Bruijn DR, Merkx GF, Veerkamp JH. Assignment of the human adipocyte fatty acid-binding protein gene (FABP4) to chromosome 8q21 using somatic cell hybrid and fluorescence in situ hybridization techniques.
• van Dongen MJ, Uppenberg J, Svensson S, Lundback T, Akerud T, Wikstrom M, Schultz J. Structure-based screening as applied to human FABP4: a highly efficient alternative to HTS for hit generation.
• Witthuhn BA, Bernlohr DA. Upregulation of bone morphogenetic protein GDF-3/Vgr-2 expression in adipose tissue of FABP4/aP2 null mice.

Note

This product is for research use only.