Angiopoietin-like Protein 3 (ANGPTL3) Human,
Sheep Polyclonal Antibody
| Other names: | ANGPTL3, ANGPTL 3, ANGPTL-3 | ||
|---|---|---|---|
| Cat. No.: |
 RD184092100 |
||
| Size: | 0.1 mg | | | |
| Cat. No.: |
RD184092100+ |
||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
|
Source of Antigen
E. coli
Host
Sheep
Preparation
The antibody was raised in sheep by immunization with the recombinant Human ANGPTL3.
Amino Acid Sequence
The Amino acid sequence of the recombinant Human ANGPTL3 is 100% homologous to the amino acid sequence of the Human ANGPTL3 (AA 17–223) without signal sequence. Total 223 AA. MW: 26 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).
MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human ANGPTL3.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
References
- Mandard S, Zandbergen F, Tan NS, Escher P, Patsouris D, Koenig W, Kleemann R, Bakker A, Veenman F, Wahli W, Muller M, Kersten S. The direct peroxisome proliferator-activated receptor target fasting-induced adipose factor (FIAF/PGAR/ANGPTL4) is present in blood plasma as a truncated protein that is increased by fenofibrate treatment. J Biol Chem. 2004 Aug 13;279(33):34411-20. Epub 2004 Jun 9.
- Koster A, Chao YB, Mosior M, Ford A, Gonzalez-DeWhitt PA, Hale JE, Li D, Qiu Y, Fraser CC, Yang DD, Heuer JG, Jaskunas SR, Eacho P. Transgenic angiopoietin-like (angptl)4 overexpression and targeted disruption of angptl4 and angptl3: regulation of triglyceride metabolism. Endocrinology. 2005 Nov;146(11):4943-50. Epub 2005 Aug 4.
Note
This product is for research use only.
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