Angiopoietin-like Protein 4 (ANGPTL4) Human,
Rabbit Polyconal Antibody
| Other names: | ANGPTL4, FIAF | ||
|---|---|---|---|
| Cat. No.: | RD181073100-01 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD181073100-01+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Source of Antigen
E. coli
Host
Rabbit
Preparation
The antibody was raised in rabbits by immunization with the recombinant Human ANGPTL4. The amino acid sequence of the recombinant Human ANGPTL4 is 100% homologous to the amino acid sequence of the Human ANGPTL4 precursor (aa 26–229) without signal sequence.
Amino Acid Sequence
Total 220 AA. MW: 25 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).
MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human ANGPTL4.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
Note
This product is for research use only.
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