Angiostatin Human,
Rabbit Polyclonal Antibody

Research topic

Oncology

Introduction to the Molecule

Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen. Recombinant angiostatin has 260 amino acids, residues 97–357 of human proplasminogen, and a calculated molecular mass of 29.8 kDa.

Note

This product is for research use only.

Source of Antigen

Human serum

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the Human Angiostatin from human plasma. The Human Angiostatin contains Kringle 1 – 4 and part of 5.

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized serum Human Angiostatin.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

Western blotting

By research topic:

• Oncology

By molecule:

• Angiostatin