Anti-ERK1/2

Introduction

Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody.

Introduction to the Molecule

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it as well. Once actived, Raf phosphorylates other two kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in ERK 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

Host

Mouse

Isotype

IgG1

Preparation

WB (with ECL): 1:1000 dilution (incubate for one hour at room temperature) For best results with other assays, please determine optimal working dilution by titration test.

Purification Method

Protein A affinity chromatography from mouse ascites fluid.

Formulation

1 mg/mL in 10 mM PBS, pH 7.2, with 0.05% NaN₃.

Storage/Stability

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks.

Applications

Western blotting

By molecule:

• ERK1/2