Anti-FLAG tag

Introduction

Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody.

Host

Mouse

Isotype

IgG2b

Preparation

WB (with ECL): 1:1000–3000 dilution (incubate for one hour at room temperature) IS: 1:500–2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.

Purification Method

Protein A affinity chromatography from mouse ascites fluid.

Formulation

1 mg/mL in 10 mM PBS, pH 7.2, with 0.05% NaN₃.

Storage/Stability

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks.

Applications

Dot, ELISA, Immunoprecipitation, IS, Western blotting

By molecule:

• FLAG Tag