Apoptotic protease activating factor-1 Human,
Mouse Monoclonal Antibody, Clone: 14DE3

Introduction to the Molecule

Apoptosis is a form of programmed cell death in multicellular organisms. Its dysfunction plays a crucial role in different human diseases, such as cancer and neurological degenerative disorders. In the process of apoptosis, a complex known as the apoptosome is formed from apoptotic protease activating factor-1 (Apaf-1), procaspase-9, and cytochrome c/dATP. The apoptosome comprises seven molecules of Apaf-1 arranged in a symmetric, wheel-shaped structure. Apaf-1 contains an N-terminal caspase recruitment domain (CARD), which is responsible for recruiting caspase-9, a nucleotidebinding oligomerization domain (NOD), and 13 WD40 repeats, which are thought to interact with cytochrome c. Oligomerization of Apaf-1 leads to autoactivation of procaspase-9 that in turn cleaves caspase-3, ultimately causing cell death. The structure of the apoptosome thus constitutes a cellular “death wheel” In many cell types, the apoptosome is dispensable for stress-induced apoptosis and it serves to amplify rather than initiate the caspase cascade and must be more important in certain cell types.

Source of Antigen

E.coli

Host

Mouse

Isotype

IgG1, gamma

Preparation

Hepes with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Species Reactivity

Human (+), Mouse (-), Rat (-)

Storage/Stability

Store for 1 year at –20°C from date of shipment

Applications

Immunoprecipitation, Western blotting

By molecule:

• Apoptotic protease activating factor-1