Beta-Microseminoprotein Human,
Sheep Polyclonal Antibody
| Other names: Prostate secretory protein PSP94, Prostate secreted seminal plasma protein, Prostate secretory protein of 94 amino acids, PSP-94, Seminal plasma beta-inhibin, Immunoglobulin-binding factor, IGBF, PN44, MSMB, PRSP | Product of BioVendor | ||||
| Product: | Size: | ||||
|---|---|---|---|---|---|
| RD184140100 | 0.1 mg | ||||
Files:
Datasheet PDFMSDS
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Product details
Research topic
Oncology
Note
This product is for research use only.
Source of Antigen
E. coli
Host
Sheep
Preparation
The antibody was raised in sheep by immunization with the recombinant Human Beta-microseminoprotein.
Amino Acid Sequence
The immunization antigen (12.01 kDa – calculated) is a protein containing 104 AA of recombinant Human
Beta-microseminoprotein. N-terminal His-tag, 10 extra AA (highlighted).
MKHHHHHHAS SCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC TCYETEISCC TLVSTPVGYD KDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human Beta-microseminoprotein.
Antibody Content
0.1 mg (determined by BCA method, BSA was used as a standard)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C.Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
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