Casein kinase 1 alpha Human,
Rabbit Polyclonal Antibody

Introduction

The casein kinase I (CKI) family of serine/threonine protein kinases is highly conserved from yeast to humans. The CKI family is involved in many diverse and important cellular functions, such as regulation of membrane transport, cell division, DNA repair, circadian rhythms, and nuclear localization. The name of the enzyme family was originated from the convenience of casein as a substrate since the earliest days of research on protein phosphorylation. Mammals have seven family members: α, β, γ1, γ2, γ3, δ, and ε. Sometimes they are referred to as isoforms but they are encoded by distinct genes. The family members appear to have similar substrate specificity in vitro and substrate selection is thought to be regulated in vivo via subcellular localization and docking sites in specific substrates. CK1α is the smallest member of the family consisting of only 325 amino acids (37.5 kDa). It can mediate phosphorylation of a wide range of proteins, including many signaling molecules. It has been suggested to play a role in phosphorylation of Disheveled in the Wnt signaling pathway.

Research topic

Immune Response, Infection and Inflammation

Introduction to the Molecule

Human Clara Cell Protein (CC16, CC10 and also called uteroglobin, urinary protein 1 or Clara Cell Secretory Protein) belongs to the family of secretoglobins and is a secreted protein product of non-ciliated bronchiolar Clara cells. Its function remains to be elucidated but there is convincing data suggesting its phospholipase A2 inhibitory activity as well as a number of other immunomodulatory features including inhibition of interferon gamma signaling and Th1 vs. Th2 lymphocyte regulation. It was proposed as a potential peripheral marker of respiratory epithelial injury and bronchial dysfunction. In serum, its increase is associated with age, asbestos, nitrogen chloride and ozone exposure, sarcoidosis and high PEEP ventilation. Decreased serum CC16 levels are found after pulmonary resection, in silica-exposed workers, smokers and in asthma. Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome).

Source of Antigen

Synthetic peptide

Host

Rabbit

Isotype

IgG

Preparation

Hepes with 0.15 M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Species Reactivity

Human (+), Mouse (+), Rat (NT)

Storage/Stability

Store for 1 year at –20℃ from date of shipment

Applications

Western blotting

By research topic:

• Immune Response, Infection and Inflammation

By molecule:

• Clara Cell Protein
• Casein kinase 1