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Cathepsin K Human,
Mouse Monoclonal Antibody, Clone: 3F9

Cat. No.: RD1820741003F9
Size: 0.1 mg |
Cat. No.: RD1820741003F9+
Size: 10 x 0.1 mg |
Files: Datasheet PDF MSDS
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Source of Antigen

E. coli

Host

Mouse

Isotype

IgG2b

Preparation

The Anti Human Cathepsin K Antibody, Clone: 3F9 is a mouse monoclonal antibody against recombinant Human Cathepsin K.

Amino Acid Sequence

The Human Cathepsin K is the part of the Human Procathepsin K. The Human Cathepsin K containing 224 amino acid residues (highlighted) of the Human Procathepsin K.

MRGSHHHHHH GSLYPEEILD THWELWKKTH RKQYNNKVDE ISRRLIWEKN LKYISIHNLE ASLGVHTYEL AMNHLGDMTS EEVVQKMTGLK VPLSHSRSND TLYIPEWEGR APDSVDYRKK GYVTPVKNQG QCGSCWAFSS VGALEGQLKK KTGKLLNLSP QNLVDCVSEN DGCGGGYMTN AFQYVQKNRG IDSEDAYPYV GQEESCMYNP TGKAAKCRGY REIPEGNEKA LKRAVARVGP VSVAIDASLT SFQFYSKGVY YDESCNSDNL NHAVLAVGYG IQKGNKHWII KNSWGENWGN KGYILMARNK NNACGIANLA SFPKM

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Immunohistochemistry, Western blotting

Note

This product is for research use only.



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