Cystatin C Human,
Rabbit Polyclonal Antibody

Source of Antigen

Human urine

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the Human Cystatin C.

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized Human Cystatin C.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References to this Product

• Danjo A, Yamaza T, Kido MA, Shimohira D, Tsukuba T, Kagiya T, Yamashita Y, Nishijima K, Masuko S, Goto M, Tanaka T . Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation. Biochem Biophys Res Commun . Aug 17;360(1):199-204 (2007)
• Nedelkov D, Nelson RW . Delineating protein-protein interactions via biomolecular interaction analysis-mass spectrometry. J Mol Recognit . Jan-Feb;16(1):9-14 (2003)
• Nedelkov D, Nelson RW . Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays. J Mol Recognit . Jan-Feb;16(1):15-9 (2003)
• Konduri SD, Yanamandra N, Siddique K, Joseph A, Dinh DH, Olivero WC, Gujrati M, Kouraklis G, Swaroop A, Kyritsis AP, Rao JS . Modulation of cystatin C expression impairs the invasive and tumorigenic potential of human glioblastoma cells. Oncogene . Dec 12;21(57):8705-12 (2002)

References

• Page M.K. et al.: . Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000) .
• Page M.K. et al. Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000) .
• Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following photothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. Epub 2005 Nov 23.
• Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. Ren Fail. 2001 May-Jul;23(3-4):431-8.
• Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y Acad Sci. 2002 Nov;977:315-21.
• Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol Dis. 2005 Feb;18(1):152-65.
• Price Ch.P., Finney H.: . Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000) .
• Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000) .
• Ono S. et al.: . Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. Acta Neurol Scand. 102, 47-52 (2000) .
• Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis.
• Nicklin M.J.H. and Barrett A.J.: . Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984) .
• Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984) .
• Lindahl P., Abrahamson M., Bjork L. Interaction of recombinant human cystatin c with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) .
• Lindahl P., Abrahamson M., Bjork L.: . Interaction of recombinant human cystatin C with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) .
• Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp Physiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24.

Note

This product is for research use only.