Ferritin H-chain Human,
Rabbit Polyclonal Antibody

Research topic

Iron metabolism

Introduction to the Molecule

Ferritin, molecular weight 450 000, is iron storage protein containing 24 units of 2 different subunits at various ratios. It contains 4 500 iron atoms in a molecule. Ferritin exist in spleen, liver, red marrow, small intestinal mucosa and muscle, and involve in iron metabolism of inner or outer cellular. Ferritin is a source of supply for metallic iron, and has a role to store iron, which catalyzes the hydroxy-radical synthesize reaction, into core of protein molecule. Determination of ferritin in serum is useful for diagnostic of disorder in iron metabolism, hepatic disease and malignant tumor.

References to summary

• Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
• Deinard AS, Sundberg RD, Lamb CM, Gonyea LM. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
• Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
• Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
• Pelletier C, Ali AM, Gauldie J, Walker WH, Luxton AW. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
• Thomas MJ, Goldie DJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
• Valdes R Jr, Brown BA, Ng RH. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
• Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980;

Source of Antigen

Synthetic peptide

Host

Rabbit

Preparation

Hepes with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Species Reactivity

Human (+), Mouse (+), Rat (-)

Storage/Stability

Store for 1 year at –20°C from date of shipment

Applications

Western blotting

References

• Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
• Deinard AS, Sundberg RD, Lamb CM, Gonyea LM. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
• Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
• Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
• Pelletier C, Ali AM, Gauldie J, Walker WH, Luxton AW. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
• Thomas MJ, Goldie DJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
• Valdes R Jr, Brown BA, Ng RH. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
• Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980;

By research topic:

• Iron metabolism

By molecule:

• Ferritin