Fibroblast Growth Factor 23 (FGF-23) Human,
Rabbit Polyclonal Antibody
| Cat. No.: | RD181109100 | ||
|---|---|---|---|
| Size: | 0.1 mg | | | |
| Cat. No.: | RD181109100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Source of Antigen
E. coli
Host
Rabbit
Preparation
The antibody was raised in rabbits by immunization with the recombinant Human FGF-23.
Amino Acid Sequence
The immunization antigen (27 kDa) is a protein containing 227 AA of recombinant Human FGF-23 and 16 additional amino acid residues – HisTag (highlighted).
MRGSHHHHHH GMASHMYPNA SPLLGSSWGG LIHLYTATAR NSYHLQIHKN GHVDGAPHQT IYSALMIRSE DAGFVVITGV MSRRYLCMDF RGNIFGSHYF DPENCRFQHQ TLENGYDVYH SPQYHFLVSL GRAKRAFLPG MNPPPYSQFL SRRNEIPLIH FNTPIPRRHT RSAEDDSERD PLNVLKPRAR MTPAPASCSQ ELPSAEDNSPM ASDPLGVVRG GRVNTHAGGT GPEGCRPFAK FI
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human FGF-23.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
Note
This product is for research use only.
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