Ghrelin Human,
Mouse Monoclonal Antibody, Clone: Ghr5

Source of Antigen

Syntetic peptide

Host

Mouse

Isotype

IgG3

Preparation

The Anti Human Ghrelin, Clone: Ghr-5 is a mouse monoclonal antibody against Human Ghrelin syntetic peptide.

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2; 0.1% SODIUM AZIDE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

SDS PAGE – to determine purity of the antibody.

Applications

ELISA, Western blotting

Note

This product is for research use only.