Hepatitis B Protein X (HBx) Virus Human,
Rabbit Polyclonal Antibody
| Other names: | HBx | ||
|---|---|---|---|
| Cat. No.: | RD981038100 | ||
| Size: | 0.05 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
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Source of Antigen
E. coli
Host
Rabbit
Preparation
The antibody was raised in rabbits by immunization with the recombinant HBx.
Amino Acid Sequence
The immunization antigen (17 kDa) is a protein containing 153 AA of recombinant HBx and one extra AA, N-terminal methionin (highlighted).
MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Hepatitis B Protein X.
Antibody Content
0.05 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Immunoprecipitation, Western blotting
References
- Song CZ, Bai ZL, Song CC, Wang QW. Aggregate formation of hepatitis B virus X protein affects cell cycle and apoptosis. World J Gastroenterol. 2003 Jul; 9(7): 1521-4.
- Hwang GY, Lin CY, Huang LM, Wang YH, Wang JC, Hsu CT, Yang SS, Wu CC. Detection of the hepatitis B virus X protein (HBx) antigen and anti-HBx antibodies in cases of human hepatocellular carcinoma. J Clin Microbiol. 2003 Dec; 41(12): 5598-603.
- Arbuthnot P, Kew M. Hepatitis B virus and hepatocellular carcinoma. Int J Exp Pathol. 2001 Apr; 82(2): 77-100. Review.
- Tralhao JG, Roudier J, Morosan S, Giannini C, Tu H, Goulenok C, Carnot F, Zavala F, Joulin V, Kremsdorf D, Brechot C. Paracrine in vivo inhibitory effects of hepatitis B virus X protein (HBx) on liver cell proliferation: an alternative mechanism of HBx-related pathogenesis. Proc Natl Acad Sci U S A. 2002 May 14; 99(10): 6991-6.
- Madden CR, Slagle BL. Stimulation of cellular proliferation by hepatitis B virus X protein. Dis Markers. 2001; 17(3): 153-7. Review.
Note
This product is for research use only.
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