HPG-1 (Human Prostate Specific Gene-1), Rabbit Polyclonal Antibody
| Other names: | HPG-1 | ||
|---|---|---|---|
| Cat. No.: | RD181061100 | ||
| Size: | 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
temporarily out of stock order in advance |
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Source of Antigen
E. coli
Host
Rabbit
Preparation
The antibody was raised in rabbits by immunization with the recombinant HPG-1.
Amino Acid Sequence
The immunization antigen (16 kDa) is a protein containing 136 AA of recombinant HPG-1. N-Terminal His-tag, 9 extra AA (highlighted).
MKHHHHHHHM IKKNLKKLGI EETYLNIIKA IYDRPTASIQ KTENLSTKTG TSQEYHLSPL WFYIVLEILA STIRQEKNLK DIHTEKEEVK LSLFADAMIL YLKKPNDSTR KLLELIKKIH SSCRIKINIH FAFCSQ
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant HPG-1.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
References
- Herness E. A. and Naz R. K. A novel human prostate-specific gene-1 (HPG-1): Molecular cloning, sequencing and its potential involvement in prostate carcinogenesis.
Note
This product is for research use only.
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