Leptin Human,
Mouse Monoclonal Antibody, Clone: LEP-02

Research topic

Animal studies, Energy metabolism and body weight regulation, Reproduction

Introduction to the Molecule

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.

Note

This product is for research use only.

Source of Antigen

E. coli

Host

Mouse

Isotype

IgG1

Preparation

The antibody is a mouse monoclonal antibody against human recombinant Human Leptin.

Species Reactivity

Human
Does not react with mouse.
Not yet tested in other species.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References

• Ricci M.R., Lee M.J., et al. Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am. J. Physiol. Endocrinol. Metab. 2005; (288), E798-E804.
• Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight reduced subjects. Nature Med. 1995; 1155 1161.
• Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155-1161 (1995) .
• Lonnqvist F., Arner P., Nordfors L. and Shalling M. Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950-953 (1995) .
• Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J. Positional Cloning of the Mouse Obese Gene and its Human Homologue. Nature 372, 425-432 (1994) .
• Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal weight and obese humans. N. Engl. J. Med. 1996; (334), 292 295.
• Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292-295 (1996) .
• Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight reducing effects of the plasma protein encoded by the obese gene. Science 1995; (269), 543 546.
• Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995) .

By research topic:

• Animal studies
• Energy metabolism and body weight regulation
• Reproduction

By molecule:

• Leptin