Leptin Receptor Human,
Mouse Monoclonal Antibody, Clone: LPR-01

Source of Antigen

NSO mouse myeloma cell line

Host

Mouse

Isotype

IgG1

Preparation

The Anti Human Leptin Receptor Antibody, Clone: LPR-01 is a mouse monoclonal antibody against recombinant Human Leptin Receptor. The Human Leptin Receptor is a recombinant protein produced in mouse myeloma cell line. DNA sequence including the extracellular domain of Leptin Receptor (amino acid residues 1 839) fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus).

Species Reactivity

Human
Does not react with mouse.
Not yet tested in other species.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Immunohistochemistry, Western blotting

Note

This product is for research use only.