Lipocalin-type prostaglandin-D synthase (Prostaglandin-H2 D-isomerase, Prostaglandin-D2 synthase, Beta-Trace Protein) Human,
Sheep Polyclonal Antibody
| Other names: | BTP, Prostaglandin-H2 D-isomerase, Lipocalin-Type Prostaglandin D Synthase, Beta-trace Protein | ||
|---|---|---|---|
| Cat. No.: |
 RD184113100 |
||
| Size: | 0.1 mg | | | |
| Cat. No.: |
RD184113100+ |
||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
|
Source of Antigen
E. coli
Host
Sheep
Preparation
The antibody was raised in sheep by immunization with the recombinant Human Beta-Trace Protein.
Amino Acid Sequence
The immunization antigen (20.3 kDa) is a protein containing 182 AA of recombinant Human Beta-Trace Protein. N-Terminal His-tag, 14 extra AA (highlighted).
MRGSHHHHHH GMASAPEAQV SVQPNFQQDK FLGRWFSAGL ASNSSWLREK KAALSMCKSV VAPATDGGLN LTSTFLRKNQ CETRTMLLQP AGSLGSYSYR SPHWGSTYSV SVVETDYDQY ALLYSQGSKG PGEDFRMATL YSRTQTPRAE LKEKFTAFCK AQGFTEDTIV FLPQTDKCMT EQ
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human Beta-Trace Protein.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2, AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
References
- Hoffmann A, Nimtz M, Conradt HS. Molecular characterization of beta-trace protein in human serum and urine: a potential diagnostic marker for renal diseases.
- Hoffmann A, Conradt HS, Gross G, Nimtz M, Lottspeich F, Wurster U. Purification and chemical characterization of beta-trace protein from human cerebrospinal fluid: its identification as prostaglandin D synthase.
Note
This product is for research use only.
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