Midkine Human,
Rabbit Polyclonal Antibody

Source of Antigen

E.coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Human Midkine.

Amino Acid Sequence

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human Midkine.

Antibody Content

0.05 mg (determined by BCA method)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Immunohistochemistry, Western blotting

References to this Product

• Dai LC, Xu DY, Yao X, Min LS, Zhao N, Xu BY, Xu ZP, Lu YL . Construction of a fusion protein expression vector MK-EGFP and its subcellular localization in different carcinoma cell lines. World J Gastroenterol . Dec 21;12(47):7649-53 (2006)

Note

This product is for research use only.