Neuroglobin Human,
Mouse Monoclonal Antibody, Clone: 13C8
| Cat. No.: | RD182043100-C8 | ||
|---|---|---|---|
| Size: | 0.1 mg | | | |
| Cat. No.: | RD182043100-C8+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Source of Antigen
E. coli
Host
Mouse
Isotype
IgG1
Preparation
The antibody is a mouse monoclonal antibody against recombinant Human Neuroglobin.
Amino Acid Sequence
The immunization antigen (17 kDa) is a protein containing 150 AA of recombinant Human Neuroglobin and one extra AA, N-terminal methionin (highlighted).
MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E
The amino acid sequence of the recombinant Human Neuroglobin is 100% homologous with the amino acid sequence of the Human Neuroglobin.
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Affinity chromatography on a column with immobilized protein G.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Immunohistochemistry, Western blotting
References
- Pesce A, Dewilde S, Nardini M, Moens L, Ascenzi P, Hankeln T, Burmester T, Bolognesi M. Human brain neuroglobin structure reveals a distinct mode of controlling oxygen affinity.
- Sun Y, Jin K, Mao XO, Zhu Y, Greenberg DA. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury.
- Sun Y, Jin K, Peel A, Mao XO, Xie L, Greenberg DA. Neuroglobin protects the brain from experimental stroke in vivo.
- Mammen PP, Shelton JM, Goetsch SC, Williams SC, Richardson JA, Garry MG, Garry DJ. Neuroglobin, a novel member of the globin family, is expressed in focal regions of the brain.
- Garry DJ, Mammen PP. Neuroprotection and the role of neuroglobin.
Note
This product is for research use only.
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