Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like) Human,
Rabbit Polyclonal Antibody

Research topic

Bone and cartilage metabolism, Cardiovascular disease, Cytokines and chemokines and related molecules

Introduction to the Molecule

Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2).

OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin

Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Note

This product is for research use only.

Source of Antigen

E.coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Human OSF-2.

Amino Acid Sequence

Species Reactivity

Human, Rat, Chicken, Mouse
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human OSF-2.

Antibody Content

0.05 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Immunohistochemistry, Western blotting

References to this Product

• Castronovo V, Waltregny D, Kischel P, Roesli C, Elia G, Rybak JN, Neri D . A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer. Mol Cell Proteomics . Nov;5(11):2083-91
• Li P, Oparil S, Novak L, Cao X, Shi W, Lucas J, Chen YF . ANP signaling inhibits TGF-beta-induced Smad2 and Smad3 nuclear translocation and extracellular matrix expression in rat pulmonary arterial smooth muscle cells. J Appl Physiol . Jan;102(1):390-8 (2007)
• Grigoriadis A, Mackay A, Reis-Filho JS, Steele D, Iseli C, Stevenson BJ, Jongeneel CV, Valgeirsson H, Fenwick K, Iravani M, Leao M, Simpson AJ, Strausberg RL, Jat PS, Ashworth A, Neville AM, O'hare MJ . Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data. Breast Cancer Rest . Oct 2;8(5):R56 (2006)
• Blumer MJ, Schwarzer C, Perez MT, Konakci KZ, Fritsch H . Identification and location of bone-forming cells within cartilage canals on their course into the secondary ossification centre. J Anat . Jun;208(6):695-707 (2006)
• Hosoya A, Nakamura H, Ninomiya T, Yoshiba K, Yoshiba N, Nakaya H, Wakitani S, Yamada H, Kasahara E, Ozawa H . Immunohistochemical localization of alpha-Smooth muscle actin during rat molar tooth development. J Histochem Cytochem . Dec;54(12):1371-8 (2006)
• Gao BB, Stuart L, Feener EP. Label-free quantitative analysis of one-dimensional PAGE LC/MS/MS proteome: application on angiotensin II-stimulated smooth muscle cells secretome. Mol Cell Proteomics. 2008 Dec;7 (12):2399-409
• Erkan M, Kleeff J, Gorbachevski A, Reiser C, Mitkus T, Esposito I, Giese T, Büchler MW, Giese NA, Friess H . Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity.
• Blanchard C, Mingler MK, McBride M, Putnam PE, Collins MH, Chang G, Stringer K, Abonia JP, Molkentin JD, Rothenberg ME. Periostin facilitates eosinophil tissue infiltration in allergic lung and esophageal responses. Mucosal Immunol. 2008 Jul;1 (4):289-96
• Kuhn B, del Monte F, Hajjar RJ, Chang YS, Lebeche D, Arab S, Keating MT. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nat Med. 2007 Aug;13 (8):962-9
• Wallace DP, Quante MT, Reif GA, Nivens E, Ahmed F, Hempson SJ, Blanco G, Yamaguchi T. Periostin induces proliferation of human autosomal dominant polycystic kidney cells through alphaV-integrin receptor. Am J Physiol Renal Physiol. 2008 Nov;295 (5):F1463-71
• Bagnato C, Thumar J, Mayya V, Hwang SI, Zebroski H, Claffey KP, Haudenschild C, Eng JK, Lundgren DH, Han DK. Proteomics analysis of human coronary atherosclerotic plaque: a feasibility study of direct tissue proteomics by liquid chromatography and tandem mass spectrometry. Mol Cell Proteomics. 2007 Jun;6 (6):1088-102
• Liang X, Zhao J, Hajivandi M, Wu R, Tao J, Amshey JW, Pope RM . Quantification of membrane and membrane-bound proteins in normal and malignant breast cancer cells isolated from the same patient with primary breast carcinoma. J Proteome Res . Oct;5(10):2632-41 (2006)
• Zhang GY, Zhang YN, Li JS, Tang WH . The expression analysis of periostin in human breast cancer tissues.
• Sun GW, Li JS, TangWH . The Expression and Significance of Periostin in Human Gastric Carcinoma.

References

• Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta.
• Takeshita S, Kikuno R, Tezuka K, Amann E. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.
• Ulstrup JC, Jeansson S, Wiker HG, Harboe M. Relationship of secretion pattern and MPB70 homology with osteoblast-specific factor 2 to osteitis following Mycobacterium bovis BCG vaccination.

By research topic:

• Bone and cartilage metabolism
• Cardiovascular disease
• Cytokines and chemokines and related molecules

By molecule:

• Osteoblast Specific Factor 2