Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like) Human,
Rabbit Polyclonal Antibody

Source of Antigen

E.coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Human OSF-2.

Amino Acid Sequence

Species Reactivity

Human, Rat, Chicken, Mouse
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human OSF-2.

Antibody Content

0.05 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Immunohistochemistry, Western blotting

References to this Product

• Castronovo V, Waltregny D, Kischel P, Roesli C, Elia G, Rybak JN, Neri D . A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer. Mol Cell Proteomics . Nov;5(11):2083-91
• Li P, Oparil S, Novak L, Cao X, Shi W, Lucas J, Chen YF . ANP signaling inhibits TGF-beta-induced Smad2 and Smad3 nuclear translocation and extracellular matrix expression in rat pulmonary arterial smooth muscle cells. J Appl Physiol . Jan;102(1):390-8 (2007)
• Grigoriadis A, Mackay A, Reis-Filho JS, Steele D, Iseli C, Stevenson BJ, Jongeneel CV, Valgeirsson H, Fenwick K, Iravani M, Leao M, Simpson AJ, Strausberg RL, Jat PS, Ashworth A, Neville AM, O'hare MJ . Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data. Breast Cancer Rest . Oct 2;8(5):R56 (2006)
• Blumer MJ, Schwarzer C, Perez MT, Konakci KZ, Fritsch H . Identification and location of bone-forming cells within cartilage canals on their course into the secondary ossification centre. J Anat . Jun;208(6):695-707 (2006)
• Hosoya A, Nakamura H, Ninomiya T, Yoshiba K, Yoshiba N, Nakaya H, Wakitani S, Yamada H, Kasahara E, Ozawa H . Immunohistochemical localization of alpha-Smooth muscle actin during rat molar tooth development. J Histochem Cytochem . Dec;54(12):1371-8 (2006)
• Erkan M, Kleeff J, Gorbachevski A, Reiser C, Mitkus T, Esposito I, Giese T, Büchler MW, Giese NA, Friess H . Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity.
• Liang X, Zhao J, Hajivandi M, Wu R, Tao J, Amshey JW, Pope RM . Quantification of membrane and membrane-bound proteins in normal and malignant breast cancer cells isolated from the same patient with primary breast carcinoma. J Proteome Res . Oct;5(10):2632-41 (2006)
• Zhang GY, Zhang YN, Li JS, Tang WH . The expression analysis of periostin in human breast cancer tissues.
• Sun GW, Li JS, TangWH . The Expression and Significance of Periostin in Human Gastric Carcinoma.

References

• Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta.
• Takeshita S, Kikuno R, Tezuka K, Amann E. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.
• Ulstrup JC, Jeansson S, Wiker HG, Harboe M. Relationship of secretion pattern and MPB70 homology with osteoblast-specific factor 2 to osteitis following Mycobacterium bovis BCG vaccination.

Note

This product is for research use only.