Phospho-p44/42 MAPK (ERK1/2) Human,
Rabbit Polyclonal Antibody

Introduction to the Molecule

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it as well. Once actived, Raf phosphorylates other two kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in ERK 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

Source of Antigen

Synthetic phospholyrated peptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1)

Host

Rabbit

Preparation

PBS containing 50% glycerol

Species Reactivity

Human (+), Mouse (+), Rat (+)

Storage/Stability

stable for 1 year at –20°C from date of shipment

Applications

Western blotting

By molecule:

• ERK1/2