Phospholipase Cγ 1 Human,
Mouse Monoclonal Antibody, Clone: 1F1

Introduction to the Molecule

The mammalian Phospholipase Cγ (PLCγ) family has two closely related proteins, PLCγ1 and PLCγ2. The PLCγ isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ catalyzes hydrolysis of phosphatidyli­nositol 4,5– bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5– triphosphate (IP3) and diacylglycerol (DAG).

Source of Antigen

E.coli

Host

Mouse

Isotype

IgG2a, kappa

Preparation

PBS containing 50% glycerol

Species Reactivity

Human (+), Mouse (+), Rat (+)

Storage/Stability

Store for 1 year at –20°C from date of shipment

Applications

Immunoprecipitation, Western blotting

By molecule:

• Phospholipase Cγ