Procathepsin K Human,
Rabbit Polyclonal Antibody
| Cat. No.: | RD181074100 | ||
|---|---|---|---|
| Size: | 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Source of Antigen
E. coli
Host
Rabbit
Preparation
The antibody was raised in rabbits by immunization with the recombinant Human Procathepsin K.
Amino Acid Sequence
The recombinant Human Procathepsin K is without signal peptid (15 amino acid residues). The immunization antigen (36.7 kDa) is a protein containing 314 amino acid residues of the Human Procathepsin K and 12 additional amino acid residues – HisTag (highlighted).
MRGSHHHHHH GSLYPEEILD THWELWKKTH RKQYNNKVDE ISRRLIWEKN LKYISIHNLE ASLGVHTYEL AMNHLGDMTS EEVVQKMTGL KVPLSHSRSN DTLYIPEWEG RAPDSVDYRK KGYVTPVKNQ GQCGSCWAFS SVGALEGQLK KKTGKLLNLS PQNLVDCVSE NDGCGGGYMT NAFQYVQKNR GIDSEDAYPY VGQEESCMYN PTGKAAKCRG YREIPEGNEK ALKRAVARVG PVSVAIDASL TSFQFYSKGV YYDESCNSDN LNHAVLAVGY GIQKGNKHWI IKNSWGENWG NKGYILMARN KNNACGIANL ASFPKM
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human Procathepsin K.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
Note
This product is for research use only.
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