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Prostate-Specific Gene-1 Human,
Rabbit Polyclonal Antibody

Other names: HPG-1 Product of BioVendor
Product: Size:
RD181061100 0.1 mg
Files: Datasheet PDFMSDS Prostate-Specific Gene-1 on pubmed

Product details

Research topic

Oncology

Introduction to the Molecule

Human Prostate-Specific Gene-1 (HPG-1) product is a recently discovered membrane-anchored protein of approximately 15 kD. It was detected exclusively in the prostate (19 different tissues were tested). Antisense-oligonicleotide inhibition of HPG-1 expression inhibited the growth of one prostate cancer cell line (LNCaP) significantly (86%). HPG-1 might be a novel diagnostic and/or therapeutic target.

Note

This product is for research use only.

References to summary

  • Herness E. A. and Naz R. K. A novel human prostate-specific gene-1 (HPG-1): Molecular cloning, sequencing and its potential involvement in prostate carcinogenesis.

Source of Antigen

E. coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant HPG-1.

Amino Acid Sequence

The immunization antigen (16 kDa) is a protein containing 136 AA of recombinant HPG-1. N-Terminal His-tag, 9 extra AA (highlighted).

MKHHHHHHHM IKKNLKKLGI EETYLNIIKA IYDRPTASIQ KTENLSTKTG TSQEYHLSPL WFYIVLEILA STIRQEKNLK DIHTEKEEVK LSLFADAMIL YLKKPNDSTR KLLELIKKIH SSCRIKINIH FAFCSQ

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant HPG-1.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

Western blotting

References

  • Herness E. A. and Naz R. K. A novel human prostate-specific gene-1 (HPG-1): Molecular cloning, sequencing and its potential involvement in prostate carcinogenesis.

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