Prothrombin precursor (coagulation factor II) Human,
Mouse Monoclonal Antibody, Clone: 28H5

Introduction to the Molecule

Prothrombin precursor is synthesized in liver as a precursor of prothrombin containing a signal sequence and a propeptide domain at the N-terminus. Before its secretion into plasma, this precursor undergoes several posttranslational modifications, including removal of signal sequence and propeptide domains, glycosylation, and γ-glutamylcarbo­xylation reaction. Prothrombin, cleaved by the prothrombinase enzyme complex that consists of serine protease factor Xa, cofactor Va, phospholipids and calcium, is converted to thrombin which converts fibrinogen into fibrin which in turn strengthens a protective clot. Activation of prothrombin is crucial in physiological and pathological coagulation. Various rare diseases involving prothrombin(e.g. hypoprothrombi­nemia) have been described.

Source of Antigen

Human plasma

Host

Mouse

Isotype

IgG2b, kappa

Preparation

Hepes with 0.15M NaCl, 0.01% BSA, 0.05% Sodium azide and 50% glycerol

Species Reactivity

Human (+), Mouse (NT), Rat (NT)

Storage/Stability

Store for 1 year at –20℃ from date of shipment

Applications

ELISA, Western blotting

By molecule:

• Prothrombin precursor