Resistin Human,
Rabbit Polyclonal Antibody

Source of Antigen

E. coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Human Resistin.

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized Human Resistin.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References to this Product

• Pravenec M, Kazdova L, Cahova M, Landa V, Zidek V, Mlejnek P, Simakova M, Wang J, Qi N, Kurtz TW . Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification. Int J Obes (Lond) . Jul;30(7):1157-9 (2006)
• Senolt L, Housa D, Vernerova Z, Jirasek T, Svobodova R, Veigl D, Anderlova K, Muller-Ladner U, Pavelka K, Haluzik M . Resistin in rheumatoid arthritis synovial tissue, synovial fluid and serum. Ann Rheum Dis . Apr;66(4):458-63 (2007)
• Senolt L, Housa D, Vernerova Z, Jirasek T, Svobodova R, Veigl D, Anderlova K, Muller-Ladner U, Pavelka K, Haluzik M . Resistin is abundantly present in rheumatoid arthritis synovial tissue, synovial fluid, and elevated serum resistin reflects disease activity. Ann Rheum Dis . Nov 22 (2006)

References

• Kim KH. et al. A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001) .
• Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA: . A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
• Steppan C.M. at al. A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502-506, (2001) .
• Way J.M. et al. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001) .
• Way JM, Görgün CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS: . Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor γ Agonists. J Biol Chem. 276, 25651-25653, (2001) .
• Banerjee RR, Lazar MA: . Dimerization of resistin and resistin–like moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001) .
• Banerjee R.R., Lazar M.A. Dimerization of resistin and resistin–like moleules is etermined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001) .
• Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T: . Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
• Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: . Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
• Hartman H.B., Lazar M.A. at al. Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002) .
• Steppan CM, Lazar MA: . Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002) .
• Steppan C.M., Lazar M.A. Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002) .
• Juan CC. at al. Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328-1333, (2001) .
• Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: . The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Steppan C.M. et al. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: . Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
• Fasshauer M. at al. Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001) .

Note

This product is for research use only.