Resistin Rat,
Rabbit Polyclonal Antibody

Research topic

Animal studies, Energy metabolism and body weight regulation

Introduction to the Molecule

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.

Note

This product is for research use only.

References to summary

• Banerjee R.R., Lazar M.A. Dimerization of resistin and resistin–like moleules is etermined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001) .
• Banerjee RR, Lazar MA:. Dimerization of resistin and resistin–like moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001) .
• Fasshauer M. at al. Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001) .
• Hartman H.B., Lazar M.A. at al. Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002) .
• Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA:. Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
• Juan CC. at al. Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328-1333, (2001) .
• Kim KH. et al. A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001) .
• Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T:. Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
• Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW:. Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
• Steppan C.M. at al. A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502-506, (2001) .
• Steppan C.M. et al. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Steppan C.M., Lazar M.A. Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002) .
• Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA:. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA:. A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
• Steppan CM, Lazar MA:. Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002) .
• Way J.M. et al. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001) .
• Way JM, Görgün CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS:. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor γ Agonists. J Biol Chem. 276, 25651-25653, (2001) .

Source of Antigen

E.coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Rat Resistin.

Amino Acid Sequence

Species Reactivity

Rat
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Rat Resistin.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References

• Banerjee R.R., Lazar M.A. Dimerization of resistin and resistin–like moleules is etermined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001) .
• Banerjee RR, Lazar MA:. Dimerization of resistin and resistin–like moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001) .
• Fasshauer M. at al. Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001) .
• Hartman H.B., Lazar M.A. at al. Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002) .
• Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA:. Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
• Juan CC. at al. Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328-1333, (2001) .
• Kim KH. et al. A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001) .
• Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T:. Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
• Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW:. Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
• Steppan C.M. at al. A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502-506, (2001) .
• Steppan C.M. et al. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Steppan C.M., Lazar M.A. Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002) .
• Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA:. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
• Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA:. A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
• Steppan CM, Lazar MA:. Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002) .
• Way J.M. et al. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001) .
• Way JM, Görgün CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS:. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor γ Agonists. J Biol Chem. 276, 25651-25653, (2001) .

By research topic:

• Animal studies
• Energy metabolism and body weight regulation

By molecule:

• Resistin