Secretagogin Rat,
Rabbit Polyclonal Antibody

Research topic

Diabetology - Autoimmunity, Diabetology - Other Relevant Products, Neural tissue damage markers, Oncology

Note

This product is for research use only.

References to summary

• Berggard T, Wagner L, James P, Lindqvist A, Linse S, Rogstam A. Binding of calcium ions and SNAP-25 to the hexa EF-hand protein secretagogin. Biochem J. 2007 Jan 1;401 (1):353-63
• Huang Q, Ren G, Xu E, Xing X, Lu B, Lai M. Secretagogin, a novel neuroendocrine marker, has a distinct expression pattern from chromogranin A. Virchows Arch. 2006 Oct;449 (4):402-9
• Preusser M, Budka H, Rossler K, Wagner L, Pipp I. Secretagogin expression in tumours of the human brain and its coverings. APMIS. 2007 Apr;115 (4):319-26
• Torring N, Orntoft TF, Birkenkamp-Demtroder K, Borre M, Ottosen P, Stattin P, Bergh A, Wagner L, Adolf K. Secretagogin is a new neuroendocrine marker in the human prostate. Prostate. 2007 Apr 1;67 (5):472-84
• Wagner L, Luger A, Majdic O, Ilhan A, Koc-Saral F, Nabokikh A, Daneva T, Vila G, Gartner W. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homolog. Am J Physiol Endocrinol Metab. 2007 Jul;293 (1):E347-54
• Wagner L, Pasternack MS, Waldhausl W, Kaserer K, Groeger M, Nowotny P, Gartner W, Oliyarnyk O. Cloning and expression of secretagogin, a novel neuroendocrine- and pancreatic islet of Langerhans-specific Ca2+-binding protein. J Biol Chem. 2000 Aug 11;275 (32):24740-51
• Wagner L, Waldhausl W, Domanovits H, Leutmetzer F, Lang W, Gartner W. Cerebral expression and serum detectability of secretagogin, a recently cloned EF-hand Ca(2+)-binding protein. Cereb Cortex. 2001 Dec;11 (12):1161-9
• Wagner L, Weissel M, Base W, Wolf F, Prayer D, Domanovits H, Leutmezer F, Sodeck G, Mineva I, Zierhut B, Gartner W. Brain natriuretic peptide correlates with the extent of atrial fibrillation-associated silent brain lesions. Clin Biochem. 2008 Dec;41 (18):1434-9

Source of Antigen

E. coli

Host

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Rat Secretagogin.

Amino Acid Sequence

Species Reactivity

Rat
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Rat Secretagogin.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C.Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

Western blotting

References

• Berggard T, Wagner L, James P, Lindqvist A, Linse S, Rogstam A. Binding of calcium ions and SNAP-25 to the hexa EF-hand protein secretagogin. Biochem J. 2007 Jan 1;401 (1):353-63
• Huang Q, Ren G, Xu E, Xing X, Lu B, Lai M. Secretagogin, a novel neuroendocrine marker, has a distinct expression pattern from chromogranin A. Virchows Arch. 2006 Oct;449 (4):402-9
• Preusser M, Budka H, Rossler K, Wagner L, Pipp I. Secretagogin expression in tumours of the human brain and its coverings. APMIS. 2007 Apr;115 (4):319-26
• Torring N, Orntoft TF, Birkenkamp-Demtroder K, Borre M, Ottosen P, Stattin P, Bergh A, Wagner L, Adolf K. Secretagogin is a new neuroendocrine marker in the human prostate. Prostate. 2007 Apr 1;67 (5):472-84
• Wagner L, Luger A, Majdic O, Ilhan A, Koc-Saral F, Nabokikh A, Daneva T, Vila G, Gartner W. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homolog. Am J Physiol Endocrinol Metab. 2007 Jul;293 (1):E347-54
• Wagner L, Pasternack MS, Waldhausl W, Kaserer K, Groeger M, Nowotny P, Gartner W, Oliyarnyk O. Cloning and expression of secretagogin, a novel neuroendocrine- and pancreatic islet of Langerhans-specific Ca2+-binding protein. J Biol Chem. 2000 Aug 11;275 (32):24740-51
• Wagner L, Waldhausl W, Domanovits H, Leutmetzer F, Lang W, Gartner W. Cerebral expression and serum detectability of secretagogin, a recently cloned EF-hand Ca(2+)-binding protein. Cereb Cortex. 2001 Dec;11 (12):1161-9
• Wagner L, Weissel M, Base W, Wolf F, Prayer D, Domanovits H, Leutmezer F, Sodeck G, Mineva I, Zierhut B, Gartner W. Brain natriuretic peptide correlates with the extent of atrial fibrillation-associated silent brain lesions. Clin Biochem. 2008 Dec;41 (18):1434-9

By research topic:

• Diabetology - Autoimmunity
• Diabetology - Other Relevant Products
• Neural tissue damage markers
• Oncology

By molecule:

• Secretagogin