Thyrostimulin Beta Subunit Human,
Sheep Polyclonal Antibody
| Other names: | Thyrostimulin beta subunit, Glycoprotein hormone beta 5, GPHB5, TSH | ||
|---|---|---|---|
| Cat. No.: |
 RD184106100 |
||
| Size: | 0.1 mg | | | |
| Cat. No.: |
RD184106100+ |
||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
|
Source of Antigen
E.coli
Host
Sheep
Preparation
The antibody was raised in sheep by immunization with the recombinant Human Thyrostimulin beta subunit.
Amino Acid Sequence
The immunization antigen (13.34 kDa) is a protein containing 120 AA of recombinant Human Thyrostimulin beta subunit. N-Terminal His-tag, 14 extra AA (highlighted).
MRGSHHHHHH GMASASSGNL RTFVGCAVRE FTFLAKKPGC RGLRITTDAC WGRCETWEKP ILEPPYIEAH HRVCTYNETK QVTVKLPNCA PGVDPFYTYP VAIRCDCGAC STATTECETI
Species Reactivity
Human
Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human Thyrostimulin beta subunit.
Antibody Content
0.1 mg (determined by BCA method)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage/Stability
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Applications
ELISA, Western blotting
References
- Okada SL, Ellsworth JL, Durnam DM, Haugen HS, Holloway JL, Kelley ML, Lewis KE, Ren H, Sheppard PO, Storey HM, Waggie KS, Wolf AC, Yao LY, Webster PJ. A glycoprotein hormone expressed in corticotrophs exhibits unique binding properties on thyroid-stimulating hormone receptor. Mol Endocrinol. 2006 Feb;20 (2):414-25
- Hsu SY, Nakabayashi K, Bhalla A. Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5. Mol Endocrinol. 2002 Jul;16 (7):1538-51
- Nakabayashi K, Matsumi H, Bhalla A, Bae J, Mosselman S, Hsu SY, Hsueh AJ. Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor. J Clin Invest. 2002 Jun;109 (11):1445-52
Note
This product is for research use only.
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