Tumor Necrosis Factor- α Human,
Mouse Monoclonal Antibody, Clone: 6C10

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Introduction to the Molecule

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Source of Antigen

E.coli

Host

Mouse

Isotype

IgG1, kappa

Preparation

Hepes with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Species Reactivity

Human (+), Mouse (NT), Rat (NT)

Storage/Stability

Store for 1 year at –20°C from date of shipment

Applications

ELISA, Western blotting

By research topic:

• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• TNF-alpha