Zinc-Alpha-2-Glycoprotein (ZA2G) Human,
Sheep Polyclonal Antibody

Source of Antigen

293 cell line (Human embryonic kidney)

Host

Sheep

Preparation

The antibody was raised in sheep by immunization with the recombinant Human ZA2G.

Amino Acid Sequence

Species Reactivity

Human
Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human ZA2G.

Antibody Content

0.1 mg (determined by BCA method)

Formulation

Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage/Stability

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody

Applications

ELISA, Western blotting

References

• Sanchez, L. M., LopezOtin, C., and Bjorkman, P. J. Biochemical characterization and crystalization of human Zn-alpha(2)-glycoprotein, a soluble class I major histocompatibility complex homolog.
• Burmeister, W. P., Gastinel, L. N., Simister, N. E., Blum, M. L., and Bjorkman, P. J. Crystal structure at 2.2 Å resolution of the MHC-related neonatal Fc receptor.
• Sanchez, L. M., Chirino, A. J., and Bjorkman, P. J. Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules.
• Bennett, M. J., Lebron, J. A., and Bjorkman, P. J. Crystal structure of the hereditary haemochromatosis protein HFE complexed with transferrin receptor.
• Im, J. S., Yu, K. O. A., Illarionov, P. A., LeClair, K. P., Storey, J. R., Kennedy, M. W., Besra, G. S., and Porcelli, S. A. Direct measurement of antigen binding properties of CD1 proteins using fluorescent lipid probes.
• Russell, S. T., and Tisdale, M. J. Effect of a tumour-derived lipid-mobilising factor on glucose and lipid metabolism in vivo.
• Gohda, T., Makita, Y., Shike, T., Tanimoto, M., Funabiki, K., Horikoshi, S., and Tomino, Y. Identification of epistatic interaction involved in obesity using the KK/Ta mouse as a type 2 diabetes model-Is Zn-alpha(2) glycoprotein-1 a candidate gene for obesity?.
• Tada, T., Ohkubo, I., Niwa, M., Sasaki, M., Tateyama, H., and Eimoto, T. Immunohistochemical localization of Zn-alpha- 2-glycoprotein in normal human tissues.
• Russell, S. T., Zimmerman, T. P., Domin, B. A., and Tisdale, M. J. Induction of lipolysis in vitro and loss of body fat in vivo by zinc-alpha(2)-glycoprotein.
• Bürgi, W., and Schmid, K. Preparation and properties of Zn-alpha- 2-glycoprotein of normal human plasma.
• Niazi, K. R., Porcelli, S. A., and Modlin, R. L. The CD1b structure: antigen presentation adapts to a high-fat diet.
• Hale, L. P. Zinc alpha-2-glycoprotein regulates melanin production by normal and malignant melanocytes.
• Bao, Y., Bing, C., Hunter, L., Jenkins, J. R., Wabitsch, M., and Trayhurn, P. Zinc-alpha(2)-glycoprotein, a lipid mobilizing factor, and is expressed and secreted by human (SGBS) adipocytes.
• Bing, C., Bao, Y., Jenkins, J., Sanders, P., Manieri, M., Cinti, S., Tisdale, M. J., and Trayhurn, P. Zinc-alpha-2-glycoprotein, a lipid-mobilizing factor, is expressed in adipocytes and upregulated in mice with cancer cachexia.
• Bing, C., Bao, Y., Jenkins, J., Sanders, P., Manieri, M., Cinti, S., Tisdale, M. J., and Trayhurn, P. Zinc-{alpha}2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia.
• Díez-Itza, I., Sánchez, L. M., Allende, M. T., Vizoso, F., Ruibal, Á., and López-Otín, C. Zn-alpha- 2-glycoprotein levels in breast cancer cytosols and correlation with clinical, histological and biochemical parameters.

Note

This product is for research use only.