17α-Hydroxyprogesterone Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

The steroid 17α−OH Progesterone is produced by the adrenal cortex and gonads. The 17α−OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21-hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21– hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17αOHP is secreted in abundant excess. Measurment of 17αOHP is therefore valuable in the initial diagnosis of CAH.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 μl

Applications

Serum

Calibration range

0 – 20 ng/ml

Limit of detection

0.11 ng/ml

Intra-assay

CV = 5.3%

Inter-assay

CV = 6.8%

Spiking Recovery

98%

Dilution Linearity

97%

References to summary

• Yeo KH, Elder PA, Lewis JG. An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate. Ann Clin Biochem. 1988 May;25 ( Pt 3):293-7
• Hofman LF, Klaniecki JE, Smith EK. Direct solid-phase radioimmunoassay for screening 17 alpha-hydroxyprogesterone in whole-blood samples from newborns. Clin Chem. 1985 Jul;31 (7):1127-30
• Thorneycroft IH, Mishell DR Jr, Stone SC, Kharma KM, Nakamura RM. The relation of serum 17-hydroxyprogesterone and estradiol-17-beta levels during the human menstrual cycle. Am J Obstet Gynecol. 1971 Dec 1;111 (7):947-51