17α-Hydroxyprogesterone ELISA

Summary

The steroid 17α−OH Progesterone is produced by the adrenal cortex and gonads. The 17α−OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21-hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21– hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17αOHP is secreted in abundant excess. Measurment of 17αOHP is therefore valuable in the initial diagnosis of CAH.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Storage/Shipping

2–8 °C

Calibration Curve

Calibration range

0.15 – 20 ng/ml

Limit of detection

0.11 ng/ml

Intra-assay

CV = 5.3%

Inter-assay

CV = 6.8%

Spiking Recovery

98%

Dilution Linearity

97%

References to summary

• Hofman LF, Klaniecki JE, Smith EK. Direct solid-phase radioimmunoassay for screening 17 alpha-hydroxyprogesterone in whole-blood samples from newborns. Clin Chem. 1985 Jul;31 (7):1127-30
• Kharma KM, Thorneycroft IH, Mishell DR Jr, Stone SC, Nakamura RM. The relation of serum 17-hydroxyprogesterone and estradiol-17-beta levels during the human menstrual cycle. Am J Obstet Gynecol. 1971 Dec 1;111 (7):947-51
• Knorr D, Bidlingmaier F, Dorr HG, Sippell WG. Plasma levels of aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyprogesterone, cortisol, and cortisone during infancy and childhood. Pediatr Res. 1980 Jan;14 (1):39-46
• Lewis JG, Elder PA, Yeo KH. An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate. Ann Clin Biochem. 1988 May;25 ( Pt 3):293-7

By research topic:

• Steroid hormones

By molecule:

• 17α-Hydroxyprogesterone

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