2 Screen Islet Cell Autoantibody Human ELISA

Summary

Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus. The antigens recognised by these autoantibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform) and an islet cell antigen named IA-2 or ICA-512. 2 Screen ELISA allows simultaneous measurement of GAD and IA- 2 autoantibodies in the same sample.

Research topic

Autoimmunity, Diabetology - Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Storage/Shipping

Sera to be analysed should be assayed soon after separation or stored, preferably in aliquots, at or below –20°C.

Calibration Curve

Calibration range

4 – 450 U/ml

Limit of detection

0.17 U/ml

Intra-assay

CV = 3.9%

Inter-assay

CV = 5.5%

References to summary

• Chen S, Willis J, Maclean C, Ananieva-Jordanova R, Amoroso MA, Brooking H, Powell M, Collins A, Bennett S, Mitchell S, Burne P, Furmaniak J, Smith BR. Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65. Clin Chim Acta. 2005 Jul 1;357 (1):74-83

By research topic:

• Autoimmunity
• Diabetology - Autoimmunity

By molecule:

• 2 Screen Islet Cell Autoantibody