5α-Androstane-3α,17β-diol Glucuronide Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

5α-Androstane-3α, 17β-diol glucuronide is a C19 steroid produced mainly as a metabolite of testosterone and dihydrotestosterone (DHT). It is largely produced in target peripheral tissues such as the skin, especially around hair follicles. The stimulation by large amounts of 3 Diol G leads to excessive hair formation, notably where hair is not normally present in women. Diabetic patients under cyclosporine A therapy have shown increased 3 Diol G levels.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 μl

Applications

Serum

Calibration range

0 – 50 ng/ml

Limit of detection

0.1 ng/ml

Intra-assay

CV = 7.0%

Inter-assay

CV = 9.2%

Spiking Recovery

94.4%

Dilution Linearity

93.8%

References to summary

• Riddick LM, Garibaldi LR, Wang ME, Senne AR, Klimah PE, Clark AT, Levine LS, Oberfield SE, Pang SY. 3 alpha-Androstanediol glucuronide in premature and normal pubarche. J Clin Endocrinol Metab. 1991 Jan;72 (1):46-50
• Pang S, MacGillivray M, Wang M, Jeffries S, Clark A, Rosenthal I, Wiegensberg M, Riddick L. 3 alpha-androstanediol glucuronide in virilizing congenital adrenal hyperplasia: a useful serum metabolic marker of integrated adrenal androgen secretion. J Clin Endocrinol Metab. 1991 Jul;73 (1):166-74
• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Jacobi GH, Wilson JD. Formation of 5alpha-androstane-3alpha,17beta-diol by normal and hypertrophic human prostate. J Clin Endocrinol Metab. 1977 Jan;44 (1):107-15
• Vexiau P, Fiet J, Boudou P, Villette JM, Feutren G, Hardy N, Julien R, Dreux C, Bach JF, Cathelineau G. Increase in plasma 5 alpha-androstane-3 alpha,17 beta-diol glucuronide as a marker of peripheral androgen action in hirsutism: a side-effect induced by cyclosporine A. J Steroid Biochem. 1990 Jan;35 (1):133-7
• Moghissi E, Ablan F, Horton R. Origin of plasma androstanediol glucuronide in men. J Clin Endocrinol Metab. 1984 Sep;59 (3):417-21
• Deslypere JP, Sayed A, Punjabi U, Verdonck L, Vermeulen A. Plasma 5 alpha-androstane-3 alpha,17 beta-diol and urinary 5 alpha-androstane-3 alpha,17 beta-diol glucuronide, parameters of peripheral androgen action: a comparative study. J Clin Endocrinol Metab. 1982 Feb;54 (2):386-91
• Reiner BJ, Donohoue PA, Migeon CJ, Berkovitz GD. Serum 3 alpha-androstanediol glucuronide measurements in sexually mature women with congenital adrenal hyperplasia during therapy. J Clin Endocrinol Metab. 1989 Jul;69 (1):105-9
• Scanlon MJ, Whorwood CB, Franks S, Reed MJ, James VH. Serum androstanediol glucuronide concentrations in normal and hirsute women and patients with thyroid dysfunction. Clin Endocrinol (Oxf). 1988 Nov;29 (5):529-38