5α-Androstane-3α,17β-diol Glucuronide ELISA

Summary

5α-Androstane-3α, 17β-diol glucuronide is a C19 steroid produced mainly as a metabolite of testosterone and dihydrotestosterone (DHT). It is largely produced in target peripheral tissues such as the skin, especially around hair follicles. The stimulation by large amounts of 3 Diol G leads to excessive hair formation, notably where hair is not normally present in women. Diabetic patients under cyclosporine A therapy have shown increased 3 Diol G levels.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Storage/Shipping

2–8 °C

Calibration Curve

Calibration range

0.25 – 50 ng/ml

Limit of detection

0.1 ng/ml

Intra-assay

CV = 7.0%

Inter-assay

CV = 9.2%

Spiking Recovery

94.4%

Dilution Linearity

93.8%

References to summary

• Berkovitz GD, Migeon CJ, Donohoue PA, Reiner BJ. Serum 3 alpha-androstanediol glucuronide measurements in sexually mature women with congenital adrenal hyperplasia during therapy. J Clin Endocrinol Metab. 1989 Jul;69 (1):105-9
• Fiet J, Boudou P, Villette JM, Feutren G, Hardy N, Julien R, Dreux C, Bach JF, Cathelineau G, Vexiau P. Increase in plasma 5 alpha-androstane-3 alpha,17 beta-diol glucuronide as a marker of peripheral androgen action in hirsutism: a side-effect induced by cyclosporine A. J Steroid Biochem. 1990 Jan;35 (1):133-7
• Jacobi GH, Wilson JD. Formation of 5alpha-androstane-3alpha,17beta-diol by normal and hypertrophic human prostate. J Clin Endocrinol Metab. 1977 Jan;44 (1):107-15
• James VH, Reed MJ, Franks S, Whorwood CB, Scanlon MJ. Serum androstanediol glucuronide concentrations in normal and hirsute women and patients with thyroid dysfunction. Clin Endocrinol (Oxf). 1988 Nov;29 (5):529-38
• Lauer CC, Ubelacker L, Check JH. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Moghissi E, Ablan F, Horton R. Origin of plasma androstanediol glucuronide in men. J Clin Endocrinol Metab. 1984 Sep;59 (3):417-21
• Riddick L, Wiegensberg M, Rosenthal I, Clark A, Jeffries S, Wang M, MacGillivray M, Pang S. 3 alpha-androstanediol glucuronide in virilizing congenital adrenal hyperplasia: a useful serum metabolic marker of integrated adrenal androgen secretion. J Clin Endocrinol Metab. 1991 Jul;73 (1):166-74
• Riddick LM, Garibaldi LR, Wang ME, Senne AR, Klimah PE, Clark AT, Levine LS, Oberfield SE, Pang SY. 3 alpha-Androstanediol glucuronide in premature and normal pubarche. J Clin Endocrinol Metab. 1991 Jan;72 (1):46-50
• Sayed A, Punjabi U, Deslypere JP, Vermeulen A, Verdonck L. Plasma 5 alpha-androstane-3 alpha,17 beta-diol and urinary 5 alpha-androstane-3 alpha,17 beta-diol glucuronide, parameters of peripheral androgen action: a comparative study. J Clin Endocrinol Metab. 1982 Feb;54 (2):386-91

By research topic:

• Steroid hormones

By molecule:

• 5α-Androstane-3α,17β-diol Glucuronide

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