8-OHdG Check Human ELISA (Multispecies specificity)

Research topic

Animal studies, Oxidative stress

Storage/Shipping

All reagents should be stored at 2–8°C. The expiry date is 12 months after manufacture. After the seal of above contents are opened, this kit should be used within 2 weeks.

Summary

8-OHdG is a widely used biomarker for the measurement of endogenous oxidative DNA damage.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 µl/well

Applications

Plasma, Serum, Tissue extract, Urine

Calibration Curve

Calibration range

0.5 to 200 ng/ml

References to summary

• Okamoto S, Ochi H: Chemical Abst. 129859a (1992)
• Kasai H, Crain PF, Kuchino Y, Nishimura S, Ootsuyama A, Tanooka H: Carcinogenesis 7, 1849–1851 (1986)
• Toyokuni S, tahala T, Hattori Y, Nishiyama Y, Yoshida A, Uchida K, Hiai H, Ochi H, Osada T: Lab.lnvest. 76, 365–374 (1997).
• Evans MD, Cooke MS, Podmore ID, Zheng Q, Herbert KE, Lunec J: Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage. Biochemical and Biophysical Research Communications 259 pp374–378 (1999).
• Shimoike T, Inoquchl T, Umeda F, Nawata H, Kawano K, Ochi H: The meaning of serum levels of advanced glycosylation end products in diabetic nephropathy. Metabolism 49(8) pp1030–1035 (2000).
• Fan WY, Opusu K, Kouda K, Nakanuta H, Satoh T, Ochi H, Takeuchi H: Reduced oxidative DNA damage by vegetable juice intake: A controlled trial. J Physiol Anthropol 19(6) pp287–289 (2000).
• Ochi H, Hashimoto M, Kurashige J: Assessment of functional tea in human using oxidative stress profile technique. Proceedings of 2001 International Conference on O-Cha (tea) Culture and Science (2001).
• Cooke MS, Evand MD, Lunec J: DNA repair: insights from urinary lesion analysis. Free Radical Research 36(9) pp929–932 (2002).
• Matsubasa T, Uchino T, Karashima S, Kondo Y, Maruyama K, Tanimura M , Endo F: Oxidative Stress in very low Birth Weight Infants As Measured by Urinary 8–0HdG. Free Radical Research 36(2) pp189–193 (2002).