8-OHdG Check (Ultrasensitive) Human ELISA (Multispecies specificity)

Summary

8-OHdG is a widely used biomarker for the measurement of endogenous oxidative DNA damage.

Research topic

Animal studies, Oxidative stress

Assay format

Competitive ELISA, Immobilized antigen

Applications

Tissue extract, Urine

Sample requirements

50 µl/well

Storage/Shipping

All reagents should be stored at 2–8°C. The kit should not be used beyond 9 months past the manufacturers date stamped on the exterior of the box.

Calibration Curve

Calibration range

0.125 to 10 ng/ml

References to summary

• Okamoto S, Ochi H: Chemical Abst. 129859a (1992)
• Kasai H, Crain PF, Kuchino Y, Nishimura S, Ootsuyama A, Tanooka H: Carcinogenesis 7, 1849–1851 (1986)
• Toyokuni S, tahala T, Hattori Y, Nishiyama Y, Yoshida A, Uchida K, Hiai H, Ochi H, Osada T: Lab.lnvest. 76, 365–374 (1997).
• Evans MD, Cooke MS, Podmore ID, Zheng Q, Herbert KE, Lunec J: Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage. Biochemical and Biophysical Research Communications 259 pp374–378 (1999).
• Shimoike T, Inoquchl T, Umeda F, Nawata H, Kawano K, Ochi H: The meaning of serum levels of advanced glycosylation end products in diabetic nephropathy. Metabolism 49(8) pp1030–1035 (2000).
• Fan WY, Opusu K, Kouda K, Nakanuta H, Satoh T, Ochi H, Takeuchi H: Reduced oxidative DNA damage by vegetable juice intake: A controlled trial. J Physiol Anthropol 19(6) pp287–289 (2000).
• Ochi H, Hashimoto M, Kurashige J: Assessment of functional tea in human using oxidative stress profile technique. Proceedings of 2001 International Conference on O-Cha (tea) Culture and Science (2001).
• Cooke MS, Evand MD, Lunec J: DNA repair: insights from urinary lesion analysis. Free Radical Research 36(9) pp929–932 (2002).
• Matsubasa T, Uchino T, Karashima S, Kondo Y, Maruyama K, Tanimura M , Endo F: Oxidative Stress in very low Birth Weight Infants As Measured by Urinary 8–0HdG. Free Radical Research 36(2) pp189–193 (2002).

By research topic:

• Animal studies
• Oxidative stress

By molecule:

• 8-OHdG