Acetylcholine Receptor Autoantibody Human ELISA

Summary

Autoantibodies to the acetylcholine receptor (AChR) are responsible for failure of the neuromuscular junction in myasthenia gravis. Measurement of these antibodies can be of considerable value in disease diagnosis and management.

Research topic

Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample requirements

25 μl

Calibration Curve

Calibration range

0.2 – 20 nmol/l

Limit of detection

0.23 nmol/l

Intra-assay

CV = 5.4 %

Inter-assay

CV = 11 %

References to summary

• Hewer R, Matthews I, Chen S, McGrath V, Evans M, Roberts E, Nute S, Sanders J, Furmaniak J, Smith BR. A sensitive non-isotopic assay for acetylcholine receptor autoantibodies. Clin Chim Acta. 2006 Feb;364 (1-2):159-66

By research topic:

• Autoimmunity

By molecule:

• Acetylcholine Receptor Autoantibody