Acrolein Adducts Human ELISA (Multispecies specificity)

Research topic

Animal studies, Others

Storage/Shipping

+4°C/ Wet ice

Summary

Acrolein is a chemical substance formed not only by combustion of petroleum, charcoal, lumber and plastic but also by cigarett smoke, exhaust gas, heating of oils and fats and it is known as a strong cytotoxic substance. It is produced by hyperoxidation of lipid and its presence as a secondary product resulting from hyperoxidation of lipid.

Assay format

Competitive ELISA, Immobilized antibody

Sample requirements

50 µl/well

Applications

Cell lysate, Tissue extract, Urine

Calibration Curve

References to summary

• Uchida K: Production of acrolein and protein modifications through lipid peroxidation. Journal of the Japanese Society of oils and Fats, 47, 1207 p, 1998.
• Uchid K et al.: Acrolein is a product of lipid peroxidation: formation of free acrolein and its conjugate with lysine residues in oxidative low density lipoproteins. J BioI.Chem., 273, 16058 p, 1998.
• Uchida K et al.: Protein-bound acrolein: Potential markers for oxidative stress. Proc, NatI.Acad,Sci­.USA,95,4882 p­, 1998.
• Calingasar CY et al.: Protein-bound acrolein: A novel marker of oxidative stress in Alzheimer's di­sease. J.Neurochem.,72,751 p, 1­999.
• Sato K et al.: A one-hour ELISA for quantitation of acrolein and hydroxynonenal-modified proteins by epltope-bound casein matrix method. Anal. Biochem., 270,323 p. 1999.