Adipocyte FABP Human ELISA

Summary

Adipocyte fatty acid binding protein AFABP is a 15 kDa member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. AFABP is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells . In mice, targeted mutations in AFABP provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from AFABP-deficient mice also have reduced efficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated AFABP expression in macrophages upon differentiation and activation. In these cells, AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE^-/- mice. These results indicate a central role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.

Features

• The total assay time is less than four hours.
• The kit measures total serum or plasma AFABP.
• Quality Controls are human serum based. No animal sera are used.

Patent Application Number: DE 10 2005 034 788.6

Research topic

Energy metabolism and body weight regulation

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Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Tissue extract

Sample requirements

10 µl/well

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.5 to 25 ng/ml

Limit of detection

Analytical Limit of Detection is calculated from the real human AFABP values in wells and is 0.1 ng/ml.

Intra-assay (Within-Run, n=8)

CV = 5.3 %

Inter-assay (Run-to-Run, n=3)

CV = 3.9 %

Spiking Recovery

105.6 %

Dilution Linearity

107.1 %

Cross-Reactivity

Cat, Dog, Monkey

References to this product

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References to summary

• Makowski L, Brittingham KC, Reynolds JM, Suttles J and Hotamisligil GS: The Fatty Acid-binding Protein, aP2, Coordinates Macrophage Cholesterol Trafficking and Inflammatory Activity. J Biol Chem. 2005 Apr 1.280(13):12888–95.
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• Lehmann F, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Lundback T, Rondahl L, Barf T: Discovery of inhibitors of human adipocyte fatty acid-binding protein, a potential type 2 diabetes target. Bioorg Med Chem Lett. 2004 Sep 6;14(17):4445–8.
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By research topic:

• Energy metabolism and body weight regulation

By molecule:

• Adipocyte Fatty Acid Binding Protein

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