Adiponectin Human ELISA (Competitive)

Research topic

Energy metabolism and body weight regulation

Features

• The total assay time is less than three hours.
• The kit measures total serum or plasma adiponectin.
• Standards are human serum based.
• Quality controls are human serum based. No animal sera are used.
• Components of the kit are ready to use (with the exception of Wash Solution).

Storage/Shipping

Store the kit at 2 8°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Summary

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells. The protein belongs to the soluble defence collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Together, these complexes make up approximately 0.01% of total serum protein. Adiponectin receptors AdipoR1 and AdipoR2 have been recently cloned; AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity. Adiponectin concentrations correlate negatively with glucose, insulin, triglyceride concentrations, liver fat content and body mass index and positively with high-density lipoprotein-cholesterol levels, hepatic insulin sensitivity and insulin-stimulated glucose disposal. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. Of particular interest is that low adiponectin serum levels predict type 2 diabetes independent of other risk factors. Adiponectin also inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. This adipokine plays a role as a scaffold of newly formed collagen in myocardial remodelling after ischaemic injury and also stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signalling in endothelial cells. Low serum adiponectin levels are found in patients with coronary artery disease. Moreover, high circulating levels of adiponectin are associated with decreased risk of myocardial infarction, independent of other factors. Altogether, adiponectin has the potential to become a clinically relevant parameter to be measured routinely in subjects at risk for type 2 diabetes, atherosclerosis and the metabolic syndrome.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

10 µl/well

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Calibration Curve

Calibration range

0.1 to 10 µg/ml

Limit of detection

Analytical Limit of Detection is calculated from the real human adiponectin values in wells and is 7ng/ml.

Limit of quantification

Assay Sensitivity takes the dilution of samples into consideration and is calculated according to the formula: Assay Sensitivity = Analytical Limit of Detection x sample dilution = 7ng/ml x 30 = 210ng/ml

Intra-assay (Within-Run, n=8)

CV = 6.7 %

Inter-assay (Run-to-Run, n=8)

CV = 5.0 %

Spiking Recovery

93 %

Dilution Linearity

95 %

Cross-Reactivity

Human, monkey

References to this product

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