ADMA ELISA

Summary

The vascular endothelium plays a central role in the regulation of vascular structure and function, mainly due to the formation of endothelium-derived nitric oxide (NO). NO is formed by the enzyme NO synthetase (NOS) from the amino acid precursor L-arginine. NOS activity can be downregulated by ADMA, an endogenous inhibitor of NOS. Elevated ADMA levels in plasma have been found in clinical studies including patients with hypercholeste­rolemia, hypertension, chronic heart failure, chronic renal failure and other internal disorders. ADMA may have diagnostic relevance as a novel cardiovascular risk marker.

Features

• For in vitro use only.
• Disposable gloves should be used.
• Material of animal origin used in the preparation of the kit has been obtained from animals certified as healthy but these materiále should be handled as potentially infectious.

Research topic

Blood pressure regulation and NO metabolism

Assay format

Competitive ELISA, Immobilized antigen

Applications

Plasma-EDTA, Serum

Sample requirements

20 μl

Storage/Shipping

Store the kit at 2–8 °C.

Calibration Curve

Calibration range

0.1 – 5.0 µmol/l

Limit of detection

0.05 μmol/l

Intra-assay

CV = 6.0 %

Inter-assay

CV = 9.5 %

Spiking Recovery

CV = 95 %

By research topic:

• Blood pressure regulation and NO metabolism

By molecule:

• ADMA