Albumin Mouse ELISA

Summary

Albumin refers generally to any protein with water solubility, which is moderately soluble in concentrated salt solutions, and experiences heat coagulation (protein denaturation). The most well-known type of albumin is the serum albumin in the blood. Serum albumin is the most abundant blood plasma protein and is produced in the liver.

Features

• This kit can measure mouse albumin with a high sensitivity (50–1000 ng/ml),
• With a small volume of sample ( 5 μl),
• With excellent reproducibility,
• And rapidly (2.5hours).

Research topic

Animal studies

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture medium, Plasma, Serum, Urine

Sample requirements

5 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

50 to 1000 ng/ml

Limit of detection

2.5 ng/ml

References to this product

• Fujii M, Inoguchi T, Maeda Y, Sasaki S, Sawada F, Saito R, Kobayashi K, Sumimoto H, Takayanagi R. Pitavastatin ameliorates albuminuria and renal mesangial expansion by downregulating NOX4 in db/db mice. Kidney Int. 2007 Aug;72 (4):473-80
• Iwashita K, Nagashima H. Rubratoxin B induces interleukin-6 secretion in mouse white adipose tissues and 3T3-L1 adipocytes. Toxicol Lett. 2008 Nov 10;182 (1-3):79-83
• Kaburagi T, Yamano T, Fukushima Y, Yoshino H, Mito N, Sato K. Effect of Lactobacillus johnsonii La1 on immune function and serum albumin in aged and malnourished aged mice. Nutrition. 2007 Apr;23 (4):342-50
• Kato H, Ishida J, Nagano K, Honjo K, Sugaya T, Takeda N, Sugiyama F, Yagami K, Fujita T, Nangaku M, Fukamizu A. Deterioration of atherosclerosis in mice lacking angiotensin II type 1A receptor in bone marrow-derived cells. Lab Invest. 2008 Jul;88 (7):731-9
• Kato K, Kosugi T, Sato W, Arata-Kawai H, Ozaki T, Tsuboi N, Ito I, Tawada H, Yuzawa Y, Matsuo S, Kadomatsu K, Maruyama S. Growth factor Midkine is involved in the pathogenesis of renal injury induced by protein overload containing endotoxin. Clin Exp Nephrol. 2011 Mar 1;
• Mita Y, Ishihara K, Fukuchi Y, Fukuya Y, Yasumoto K. Supplementation with chromium picolinate recovers renal Cr concentration and improves carbohydrate metabolism and renal function in type 2 diabetic mice. Biol Trace Elem Res. 2005 Summer;105 (1-3):229-48
• Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K, Kume S. Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells. 2008 Jul;13 (7):731-46
• Soto-Gutierrez A, Navarro-Alvarez N, Rivas-Carrillo JD, Tanaka K, Chen Y, Misawa H, Okitsu T, Noguchi H, Tanaka N, Kobayashi N. Construction and transplantation of an engineered hepatic tissue using a polyaminourethane-coated nonwoven polytetrafluoroethylene fabric. Transplantation. 2007 Jan 27;83 (2):129-37
• Soto-Gutierrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J, Tanaka N, Fox IJ, Kobayashi N. Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Nat Protoc. 2007;2 (2):347-56
• Takeuchi T, Ochiya T, Takezawa T. Tissue array substratum composed of histological sections: a new platform for orienting differentiation of embryonic stem cells towards hepatic lineage. Tissue Eng Part A. 2008 Feb;14 (2):267-74
• Tsutsui M, Ogawa S, Inada Y, Tomioka E, Kamiyoshi A, Tanaka S, Kishida T, Nishiyama M, Murakami M, Kuroda J, Hashikura Y, Miyagawa S, Satoh F, Shibata N, Tagawa Y. Characterization of cytochrome P450 expression in murine embryonic stem cell-derived hepatic tissue system. Drug Metab Dispos. 2006 Apr;34 (4):696-701
• Tsutsui M, Ogawa S, Inada Y, Tomioka E, Kamiyoshi A, Tanaka S, Kishida T, Nishiyama M, Murakami M, Kuroda J, Hashikura Y, Miyagawa S, Satoh F, Shibata N, Tagawa Y. Characterization of cytochrome P450 expression in murine embryonic stem cell-derived hepatic tissue system. Drug Metab Dispos. 2006 Apr;34 (4):696-701

By research topic:

• Animal studies

By molecule:

• Albumin