Aldosterone ELISA

Summary

Aldosterone is a potent mineral corticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Measurement of aldosterone levels in serum in conjunction with plasma renin levels can be used to differentiate between primary and secondary aldosteronism.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Plasma, Serum, Urine

Sample requirements

50 μl

Calibration Curve

Calibration range

0 – 2000 pg/ml

Limit of detection

15 pg/ml

Intra-assay

CV = 6.5%

Inter-assay

CV = 8.7%

Spiking Recovery

106.5 %

Dilution Linearity

97.1 %

References to summary

• Lun S, Espiner EA, Nicholls MG, Yandle TG. A direct radioimmunoassay for aldosterone in plasma. Clin Chem. 1983 Feb;29 (2):268-71
• Cartledge S, Lawson N. Aldosterone and renin measurements. Ann Clin Biochem. 2000 May;37 ( Pt 3):262-78
• Stabler TV, Siegel AL. Chemiluminescence immunoassay of aldosterone in serum. Clin Chem. 1991 Nov;37 (11):1987-9
• Oelkers W, Diederich S, Bahr V. Diagnosis and therapy surveillance in Addison's disease: rapid adrenocorticotropin (ACTH) test and measurement of plasma ACTH, renin activity, and aldosterone. J Clin Endocrinol Metab. 1992 Jul;75 (1):259-64
• Sequeira SJ, Loughlin T, Cunningham S, Culliton MT, Hannon S, Heffernan A, McKenna TJ. Evaluation of an aldosterone radioimmunoassay: the renin-angiotensin-aldosterone axis as a function of sex and age. Ann Clin Biochem. 1986 Jan;23 ( Pt 1):65-75
• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Varsano-Aharon N, Ulick S. Further simplifications in the immunoassay of plasma aldosterone. J Clin Endocrinol Metab. 1974 Aug;39 (2):375-9
• Al-Dujaili EA, Edwards CR. Optimization of a direct radioimmunoassay for plasma aldosterone. J Steroid Biochem. 1981 May;14 (5):481-7
• Himathongkam T, Dluhy RG, Williams GH. Potassim-aldosterone-renin interrelationships. J Clin Endocrinol Metab. 1975 Jul;41 (1):153-9
• Vallotton MB. Primary aldosteronism. Part I. Diagnosis of primary hyperaldosteronism. Clin Endocrinol (Oxf). 1996 Jul;45 (1):47-52
• Corry DB, Tuck ML. Secondary aldosteronism. Endocrinol Metab Clin North Am. 1995 Sep;24 (3):511-29

By research topic:

• Steroid hormones

By molecule:

• Aldosterone